acceptable dna concentrations  (Thermo Fisher)

Journal: Synthetic and Systems Biotechnology

Article Title: Metabolic engineering enables Escherichia coli to grow on 1,3-propanediol

doi: 10.1016/j.synbio.2025.11.009

Figure Lengend Snippet: Strain verification and ALE of strain PA0. (a) Shows the representation of the frequency of each of individual read from the genetic material extracted from PA1 as a plot diagram. It can be concluded that genetic material extracted from PA1 comprise of the host genomic DNA which belongs to E. coli MG1655Δ and pPAD and pBA06, proving that the mutant strain was not a contamination of a foreign bacteria species. (b) and (c) track the 1,3-PDO consumption and growth profiles of significant passages during the ALE of PA0 . PA16 , the fastest growing strain could consume 6.5 g/L of 1,3-PDO to achieve an OD 600 of 7 within 72 h. (d) An overlay of chromatograms of the supernatant collected from culturing PA16 . The two peaks on the left represents the salts present in all samples. The retention time of 1,3-PDO is 14.82 min as shown. The decline in concentration of 1,3-PDO across 72 h are shown here in blue (24 h), green (48 h) and purple (72 h). Also shown in the chromatogram, no intermediates were observed to be accumulated.

Article Snippet: After verification of DNA concentrations using Nanodrop the samples were sequenced using an Illumina platform through a third-party service provider (NovogeneAIT Genomics Singapore).

Techniques: Mutagenesis, Bacteria, Concentration Assay